Subtle Intricacies Identified during Streptozotocin-Induced Diabetes in Wistar Rats

Abstract: Aim: This study aimed to establish the dose of streptozotocin required induce Type -1 diabetes in Wistar rats. Background: Streptozotocin is currently employed worldwide insulin-dependent mellitus also known as 1 experimental animals. Though many reports on use induction are reported literature, they were not reproducible. Moreover, there was no mention mortality associated with same well conditions followed during study. Materials and Methods: In present study, dose, route, solvent used for investigated. Various doses 55, 50, 40, 35mg/kg dissolved either freshly prepared cold 10mM sodium citrate buffer (pH 4.5) or normal saline solution administered intraperitoneally intravenously. For intraperitoneal administration, dose-volume 10mL/kg whereas intravenous administration 2mL/Kg. Different routes ascertain cause which an autopsy performed. Besides rate determined. Results Discussion: The findings revealed that a 35-40mg/kg showed less percentage successful diabetes. Conclusion: Mortality (10-20%) observed at streptozotocin. Therefore, (35-40mg/kg) confirmed be safe effective paper highlights subtle intricacies rats using Keywords: Male rats, Streptozotocin, Type-1 diabetes, 5% dextrose, Mortality, Normal solution.